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| Silva,Karla Patrícia Chaves da; Takaki,Galba Maria de Campos; Silva,Leonildo Bento Galiza da; Saukas,Tomoe Noda; Santos,André Souza; Mota,Rinaldo Aparecido. |
| To assess the potency of the PPD-mallein produced in Brazil, five animals were from a property identified as a focus of glanders. These animals had suggestive clinical signs of the disease and the other five, from a property free from glanders, showed no clinical signs and were serology negative (control group). PPD-mallein from Burkholderia mallei was obtained by precipitation with trichlo-roa-cetic acid and ammonium sulfate. The animals were inoculated according to the criteria established by Department of Agriculture, Livestock and Supply (MAPA) for the diagnosis of glanders. After 48 h of application of PPD-mallein, there was swelling in the area of application, presence of ocular secretion and tears in sick animals. The control group showed no... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Mallein test; Equus asinus; Diagnosis. |
| Ano: 2013 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000100027 |
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| Souza,Patrícia Mendes de; Marinho,Petrusk Homero; Lima,Marcos Antônio Barbosa de; Nascimento,Aline Elesbão do; Takaki,Galba Maria de Campos. |
| The aim of this work was to evaluate the physiological aspects of polyphosphate metabolism of Cunninghamella elegans grown in presence of copper. The growth profile was obtained by means of biomass yields, orthophosphate consumption, polyphosphate accumulation and phosphatases activities. The results revealed the influence of copper on the growth, observed by biomass yields. Orthophosphate consumption was faster in cells grown in the presence of copper. The presence of copper in the culture medium induced polyphosphate accumulation. The polyphosphate level was almost constant in the beginning of control culture growth, and could be related to the exponential growth phase. On the other hand, the copper treated cultures exhibited a significant reduction in... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Cunninghamella elegans; Copper; Polyphosphate; Phosphatases. |
| Ano: 2005 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822005000400002 |
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| Donato,Virgínia Maria Tenório Sabino; Andrade,Arnóbio Gonçalves de; Takaki,Galba Maria de Campos; Mariano,Rosa de Lima Ramos; Maciel,Gabriel Alves. |
| A contaminação por microrganismos é conhecida como um dos mais sérios problemas da cultura de tecidos de plantas, especialmente, em espécies tropicais. Com o objetivo de eliminar bactérias endofíticas, propágulos das cultivares de cana-de-açúcar Co 997, SP 70-1143 e ápices caulinares isolados de plantas, provenientes de semente botânica biparental (RB 818004 x SP 71-6949), foram cultivados in vitro, em meios de cultivo com antibióticos. No primeiro experimento, propágulos das cultivares Co 997 e SP 70-1143 foram cultivados em meio de cultivo contendo 200 e 300 mg.L-1 de amoxicilina e cefatoxima sódica durante 30 dias. Num segundo experimento, utilizou-se apenas a amoxicilina em concentrações mais elevadas ( 300, 600 e 1000 mg.L-1), com as mesmas plantas... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Contaminação; Bactérias; Acetobacter diazotrophicus; Saccharum sp. |
| Ano: 2005 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-70542005000100017 |
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